Phospho-MLKL (S345) Recombinant Rabbit Monoclonal Antibody [JM92-37]

Phospho-MLKL (S345) Recombinant Rabbit Monoclonal Antibody [JM92-37]

• Description: Mixed lineage kinase domain like pseudokinase (MLKL) is a protein that in humans is encoded by the MLKL gene. This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children.
• Product Name Alternative: 9130019I15Rik antibody FLJ34389 antibody hMLKL antibody Mixed lineage kinase domain like antibody Mixed lineage kinase domain like protein antibody Mixed lineage kinase domain like pseudokinase antibody Mixed lineage kinase domain-like protein antibody Mlkl antibody MLKL_HUMAN antibody
• Abbreviation: Mlkl
• CAS Number: 9000-83-3
• Swiss Prot: Q9D2Y4 Mouse
• Cellular Locus: Cell membrane, Cytoplasm, Nucleus.
• Host: Rabbit
• Species Reactivity: Mouse
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser345 of mouse MLKL aa 314-363 / 472.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, IHC-Fr
• Positive Control: L929 treated with 20μM Z-VAD for 3.5 hours then added 100nM SM-164 and 20ng/ml TNF-α for 3 hours cell lysate, mouse spleen tissue, mouse lung tissue, mouse liver tissue, mouse colon tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000 ;IHC-P: 1:50-1:100 ;IHC-Fr: 1:100
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 54 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes