ERK2 Antibody, KO Validated

• Background:

  This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.

• NCBI Gene ID:

  5594

• Swiss Prot:

  P28482

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ERK2 (NP_620407.1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Antibody is Knockout validated.

• Field of Research:

  Apoptosis, Cell Cycle, Immunology, Neuroscience, Signal Transduction, Stem Cell

• Purification:

  Affinity purification

• Positive Control:

  HeLa

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 41kDa

• Fragment:

  IgG

• Symbol:

  MAPK1

• Positive Control 2:

  Jurkat

• Positive Control 3:

  Mouse brain

• Positive Control 4:

  Mouse spleen

• Positive Control 5:

  Rat brain

• Positive Control 6:

  Rat spinal cord

• NCBI Official Name:

  Mitogen-activated protein kinase 1

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  MAPK1, ERK, p38, p40, p41, ERK2, ERT1, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk, mitogen-activated protein kinase 1, protein tyrosine kinase ERK2, mitogen-activated protein kinase 2, extracellular signal-regulated kinase 2

• Tested Applications:

  WB, IHC

• Physical Properties:

  Liquid