NCOA4 (IN) Antibody

• Background:

  NCOA4 Antibody: Study showed that NCOA4-mediated ferritin complex degradation supplies iron to mitochondria. Loss of COPZ1 induces NCOA4 mediated autophagy and ferroptosis in glioblastoma cell lines. NCOA4-mediated ferritinophagy promoted inflammatory responses in periodontitis. Low expression of ferritinophagy-related NCOA4 gene in relation to unfavorable outcome and defective immune cells infiltration in clear cell renal carcinoma.

• NCBI Gene ID:

  8031

• Swiss Prot:

  Q13772

• Accession Number:

  Q13772

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  9663

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  NCOA4 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1202 – Daudi Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: monkey (100%), gorilla (100%), dog (100%), horse (100%)

• Buffer:

  NCOA4 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  NCOA4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Applications Notes:

  WB: 0.5-1 μ g/mL; IHC: 1 μ g/mL; IF: 20 μ g/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples and Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.

• Isoform:

  Human NCOA4 has 4 isoforms, including isoform alpha (614aa, 69.7kD), isoform beta (287aa, 32.6kD), isoform 3 (630aa, 71.4kD) and isoform 4 (650aa, 73.6kD) . Mouse NCOA4 has at least one isoform (625aa, 70.4kD) . Rat NCOA4 has one isoform (622aa, 70.3kD) . 9663 can detect NCOA4 in human, mouse and rat.

• Symbol:

  NCOA4

• Positive Control 2:

  Cat. No. 1402 – Mouse Lung Lysate

• NCBI Official Name:

  Nuclear receptor coactivator 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Fujimaki et al. Mol Cell Biol. 2019; 39 (14) : e00010-19

• Background Reference 02:

  Zhang et al. Oncogene. 2021; 40 (8) : 1425-1439

• Background Reference 03:

  Guo et al. J Periodontal Res. 2021; 56 (3) : 523-534

• Background Reference 04:

  Mou et al. BMC Cancer. 2021; 21 (1) :18

• Other Product Names:

  NCOA4 Antibody: Nuclear receptor coactivator 4, NCoA-4, Androgen receptor coactivator 70 kDa protein, 70 kDa AR-activator, 70 kDa androgen receptor coactivator, Androgen receptor-associated protein of 70 kDa, Ret-activating protein ELE1, ARA70, ELE1, RFG

• Tested Applications:

  ELISA, IHC, WB, IF

• Protein ID:

  8928226

• Physical Properties:

  Liquid