DEPDC1B Antibody

• Background:

  The DEP domain-containing 1B (DEPDC1B) protein was initially identified as a radiation response biomarker in human lymphoblastoid cell lines (1) . Like the related protein DEPDC1A, expression of DEPDC1B correlates with a poor prognosis in cancer patients (2,3) . Studies have shown that DEPDC1B levels are upregulated in non-small cell lung cancer (NSCLC) and have an inverse correlation with patient survival. Ectopic expression of DEPDC1B in NSCLC activated Wnt/B-catenin signaling and enhanced cell migration and invasion while silencing DEPDC1B expression suppressed these traits (2) .

• NCBI Gene ID:

  55789

• Swiss Prot:

  Q8WUY9

• Accession Number:

  NP_060839

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  DEPDC1B antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1204 - K562 Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse: (94%), Chicken: (88%)

• Buffer:

  DEPDC1B antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  DEPDC1B antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment:

  IgG

• Specificity:

  DEPDC1B antibody is human specific. At least two isoforms are known to exist; this antibody will only detect the largest isoform. DEPDC1B antibody is predicted to not cross-react with DEPDC1A.

• Symbol:

  DEPDC1B

• Positive Control 2:

  Cat. No. 10-901 - Human Spleen Tissue Slide

• NCBI Official Name:

  DEP domain containing 1B

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Niu N, Qin Y, Fridley BL, et al. Radiation pharmacogenomics: a genome-wide association approach to identify radiation response biomarkers using human lymphoblastoid cell lines. Genome Res. 2010; 20:1482-92.

• Background Reference 02:

  Yang Y, Liu L, Cai J, et al. DEPDC1B enhances migration and invasion of non-small cell lung cancer cells via activating Wnt/B-catenin signaling. Biochem. Biophys. Res. Commun. 2014; 450:899-905.

• Background Reference 03:

  Kassambara A, Schoenhals M, Moreaux J, et al. Inhibition of DEPDC1A, a bad prognostic marker in multiple myeloma, delays growth and induces mature plasma cell markers in malignant plasma cells. PLoS One 2013; 8:e62752.

• Other Product Names:

  DEP domain-containing 1B, DEP1B, BRCC3, Breast cancer cell 3, XTP1

• Tested Applications:

  ELISA, WB, IHC-P

• Protein ID:

  223633999

• Physical Properties:

  Liquid