Hexokinase 1 Antibody

• Background:

  There are four major glucose-phosphorylating isoenzymes, designated Hexokinase 1 I, II, III, and IV (1) . Hexokinase 1 activity is involved in the first step in several metabolic pathways including phosphorylation of glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway (1,2) . Hexokinase 1 2 is the predominant Hexokinase 1 isozyme expressed in insulin-responsive tissues such as skeletal muscle and its expression is insulin-responsive (3) . It is involved in the increased rate of glycolysis seen in rapidly growing cancer cells (4) .

• NCBI Gene ID:

  3098

• Swiss Prot:

  P19367

• Accession Number:

  NP_000179

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  Hexokinase 1 antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1478 - Rat Bladder Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (94%)

• Buffer:

  Hexokinase 1 antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Hexokinase 1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment:

  IgG

• Specificity:

  Hexokinase 1 antibody is human, mouse and rat reactive. Multiple isoforms of Hexokinase 1 are known to exist.

• Symbol:

  HK1

• NCBI Official Name:

  Hexokinase 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Chen T, Ning D, Sun H, et al. Sequence Analysis and Molecular Characterization of Clonorchis sinensis Hexokinase 1, an Unusual Trimeric 50-kDa Glucose-6-Phosphate-Sensitive Allosteric Enzyme. PLoS One 2014; 9:e107940.

• Background Reference 02:

  Halestrap AP, Pereira GC, and Pasdois P. The role of Hexokinase 1 in cardioprotection-mechanism and potential for translation. Br. J. Pharmacol. 2014; epub.

• Background Reference 03:

  Roberts DJ and Miyamoto S. Hexokinase 1 II integrates energy metabolism and cellular protection: Akting on mitochondria and TORCing to autophagy. Cell Death Differ. 2014; epub.

• Background Reference 04:

  Wang L, Xiong H, Wu F, et al. Hexokinase 1 2-Mediated Warburg Effect Is Required for PTEN- and p53-Deficiency-Driven Prostate Cancer Growth. Cell Rep. 2014; 8:1461-74.

• Other Product Names:

  HK, HXK

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  188497754

• Physical Properties:

  Liquid