HMGB1 Antibody

• Background:

  The high-mobility group box 1 (HMGB1) protein was originally identified as a highly conserved nuclear DNA-binding protein that participates in DNA replication, repair and transcriptional regulation of gene expression (1,2) . However, HMGB1 has more recently emerged as an extracellularly released mediator of inflammation and repair responses in lipopolysaccharide (LPS) -induced endotoxemia and sepsis (3) . HMGB1 has also been suggested to induce secretion of pro-inflammatory cytokines and chemokines by inducing NF-kB signaling downstream of Toll-like receptor (TLR) 2 and TLR4 activation (4) .

• NCBI Gene ID:

  3146

• Swiss Prot:

  P09429

• Accession Number:

  NP_002119

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  7715

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  KO Validation (Figure 1) : Anti-HMGB1 antibodies (7715) specificity was further verified by HMGB1 specific knockout cell lysate. HMGB1 signal was detected in wild type cells, but not in the HMGB1 knockout cells. KO Validation7715)

• Field of Research:

  Innate Immunity

• Purification:

  HMGB1 antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1303 - Human Brain Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Chicken: (100%), Pig: (100%)

• Buffer:

  HMGB1 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  HMGB1 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  HMGB1 antibody is human, mouse and rat reactive.

• Isoform:

  Human HMGB1 has only 1 isoform (215aa, 25kD) . Mouse HMGB1 has only 1 isoform (215aa, 25kD) . Rat HMGB1 has only 1 isoform (215aa, 25kD) . This antibody detects HMGB1 of human, mouse and rat.

• Symbol:

  HMGB1

• Positive Control 2:

  Cat. No. 10-101 - Human Lung Tissue Slide

• NCBI Official Name:

  High mobility group box 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Smerdon MJ and Isenberg I. Interactions between the subfractions of calf thymus H1 and nonhistone chromosomal proteins HMG1 and HMG2. Biochemistry 1976; 15:4242-7.

• Background Reference 02:

  Stros. Biocim. Biophys. Acta. 2010; 1799:101-113.

• Background Reference 03:

  Wang et al. Science 1999; 285:248-51.

• Background Reference 04:

  Van Zoelenet al. Shock 2009; 31:280-4.

• Background Reference 05:

  Wei etal. Cell 2021; 184:76-91.

• Other Product Names:

  HMGB1 Antibody: HMG1, HMG3, SBP-1, HMG1, High mobility group protein B1, High mobility group protein 1, HMG-1

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  4504425

• Physical Properties:

  Liquid