PHC3 Antibody

• Background:

  Polycomb group (PcG) proteins assemble into multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes throughout development (1) . PHC3 (Polyhomeotic-like protein 3), also known as Early development regulatory protein 3, is a 983 amino acid nuclear protein that is a component of the PcG multiprotein PRC1 complex (2) . PRC1 complex acts via chromatin remodeling and modification of histones. PHC3 contains 1 FCS-type zinc finger and SAM (sterile alpha motif) domain (2-3) . PHC 3 has been regarded as a candidate tumor suppressor of osteosarcoma (4) .

• NCBI Gene ID:

  80012

• Swiss Prot:

  Q8NDX5

• Accession Number:

  NP_079223

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Stem Cell

• Purification:

  PHC3 antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1282 - 3T3 (NIH) Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  PHC3 antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PHC3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Fragment:

  IgG

• Specificity:

  PHC3 antibody is human, mouse and rat specific. At least six isoforms of PHC3 are known to exist.

• Symbol:

  PHC3

• NCBI Official Name:

  Polyhomeotic homolog 3 (Drosophila)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Valk-Lingbeek ME, Bruggeman SW, and Van Lohuizen M. Stem cells and cancer; the polycomb connection. Cell 2004; 118:409–18.

• Background Reference 02:

  Deshpande AM, Akunowicz JD, Reveles XT, et al. PHC3, a component of the hPRC-H complex, associates with E2F6 during G0 and is lost in osteosarcoma tumors. Oncogene 2007; 26:1714-22.

• Background Reference 03:

  Isono K, Fujimura Y, Shinga J, et al. Mammalian polyhomeotic homologues Phc2 and Phc1 act in synergy to mediate polycomb repression of Hox genes. Mol. Cell Biol. 2005; 25:6694-706.

• Background Reference 04:

  Iwata S, Takenobu H, Kageyama H, et al. Polycomb group molecule PHC3 regulates polycomb complex composition and prognosis of osteosarcoma. Cancer Sci. 2010; 101:1646-52.

• Other Product Names:

  PHC3 Antibody: EDR3, HPH3, EDR3, PH3, Polyhomeotic-like protein 3, Early development regulatory protein 3, hPH3

• Tested Applications:

  ELISA, WB

• Protein ID:

  148612879

• Physical Properties:

  Liquid