PTRF Antibody

• Background:

  PTRF Antibody: The polymerase I and transcript release factor (PTRF) enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts, regulating rRNA transcription by promoting the dissociation of transcription complexes and the re-initiation of polymerase I on nascent rRNA transcripts. PTRF also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins, translocating from caveolae to the cytoplasm after insulin stimulation. PTRF is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy.

• NCBI Gene ID:

  284119

• Swiss Prot:

  Q6NZI2

• Accession Number:

  NP_036364

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  PTRF Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1306 - Human Spleen Tissue Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat: (95%), Mouse: (95%)

• Buffer:

  PTRF Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PTRF antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

• Calculated Molecular Weight:

  43 kDa

• Fragment:

  IgG

• Specificity:

  At least three isoforms of PTRF are known to exist; this antibody detects all three isoforms. It is predicted to not cross-react with other members of the cavin family.

• Symbol:

  PTRF

• Positive Control 2:

  Cat. No. 10-901 - Human Spleen Tissue Slide

• NCBI Official Name:

  Polymerase I and transcript release factor

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Jansa P, Mason SW, Hoffmann-Rohrer U, et al. Cloning and functional characterization of PTRF, a novel protein which induces dissociation of paused ternary transcription complexes. EMBO J. 1998; 17:2855-64.

• Background Reference 02:

  Vinten J, Johnsen AH, Roepstorff P, et al. Identification of a major protein on the cytosolic face of caveolae. Biochim. Biophys. Acta 2005; 1717:34-40.

• Background Reference 03:

  Aboulaich N, Ortegren U, Vener AV, et al. Association and insulin regulated translocation of hormone-sensitive lipase with PTRF. Biochem. Biophys. Res. Commun. 2006; 350:657-61.

• Background Reference 04:

  Aboulaich N, Chui PC, Asara JM, et al. Polymerase I and transcript release factor regulates lipolysis via a phosphorylation-dependent mechanism. Diabetes 2011; 60:757-65.

• Other Product Names:

  PTRF Antibody: CGL4, CAVIN, CAVIN1, FKSG13, cavin-1, Polymerase I and transcript release factor, Cavin-1

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  42734430

• Physical Properties:

  Liquid