PHLPP1 Antibody

• Background :

  PHLPP1 Antibody: PHLPP1 is a member of the serine/threonine phosphatase family, which are important regulators of Akt serine-threonine kinases (AKT1, AKT2, AKT3) and conventional/novel protein kinase C (PKC) isoforms. PHLPP1 and PHLPP2 have a similar domain structure and have been shown to dephosphorylate and inactivate, distinct Akt isoforms, at one of the two critical phosphorylation sites required for activation: Serine473. PHLPP2 dephosphorylates AKT1 and AKT3, whereas PHLPP1 is specific for AKT2 and AKT3. PHLPP1 promotes apoptosis and may act as a tumor suppressor. Increased expression of PHLPP1 may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.

• NCBI Gene ID :

  23239

• Swiss Prot :

  O60346

• Accession Number :

  NP_919431

• Host :

  Rabbit

• Reactivity :

  Human

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Apoptosis

• Purification :

  PHLPP1 Antibody is affinity chromatography purified via peptide column.

• Positive Control :

  Cat. No. 1221 - SW480 Cell Lysate

• Concentration :

  1 mg/mL

• Buffer :

  PHLPP1 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  PHLPP1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  189 kDa

• Fragment :

  IgG

• Specificity :

  At least four isoforms are known to exist; this antibody will only detect the largest isoform. PHLPP1 antibody is predicted to not cross react with PHLPP2.

• Symbol :

  PHLPP1

• NCBI Official Name :

  PH domain and leucine rich repeat protein phosphatase 1

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Brognard J and Newton AC. PHLiPPing the switch on Akt and protein kinase C signaling. Trends Endocrinol. Metab. 2008; 19:223-30.

• Background Reference 02 :

  Gao T, Furnari F and Newton AC. PHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth. Mol. Cell 2005; 18:13-24.

• Background Reference 03 :

  Brognard J, Sierecki E, Gao T, et al. PHLPP and a second isoform, PHLPP2, differentially attenuate the amplitude of Akt signaling by regulating distinct Akt isoforms. Mol. Cell 2007; 25:917-31

• Background Reference 04 :

  Andreozzi F, Procopio C, Greco A, et al. Increased levels of the Akt-specific phosphatase PH domain leucine-rich repeat protein phosphatase (PHLPP) -1 in obese participants are associated with insulin resistance. Diabetologia 2011; 54:1879-87.

• Other Product Names :

  PHLPP1 Antibody: SCOP, PHLPP, PLEKHE1, KIAA0606, SCOP, PH domain leucine-rich repeat-containing protein phosphatase 1, Pleckstrin homology domain-containing family E member 1, PH domain-containing family E member 1

• Tested Applications :

  ELISA, WB, ICC, IF

• Protein ID :

  291219891

• Physical Properties :

  Liquid