PHLPP2 Antibody

• Background:

  PHLPP2 Antibody: PHLPP2 is a member of the serine/threonine phosphatase family, which are important regulators of Akt serine-threonine kinases (AKT1, AKT2, AKT3) and conventional/novel protein kinase C (PKC) isoforms. PHLPP1 and PHLPP2 have a similar domain structure and have been shown to dephosphorylate and inactivate, distinct Akt isoforms, at one of the two critical phosphorylation sites required for activation: Serine473. PHLPP2 dephosphorylates AKT1 and AKT3, whereas PHLPP1 is specific for AKT2 and AKT3. PHLPP1 promotes apoptosis and may act as a tumor suppressor. PHLPP2 associates with and is inhibited by adenylyl cyclase type 6 (AC6), thereby allowing Akt activation.

• NCBI Gene ID:

  23035

• Swiss Prot:

  Q6ZVD8

• Accession Number:

  NP_055835

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis

• Purification:

  PHLPP2 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1221 - SW480 Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  PHLPP2 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PHLPP2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  156 kDa

• Fragment:

  IgG

• Specificity:

  At least three isoforms are known to exist; this antibody will detect the two largest isoforms. PHLPP2 antibody is predicted to not cross react with PHLPP1.

• Symbol:

  PHLPP2

• NCBI Official Name:

  PH domain and leucine rich repeat protein phosphatase 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Brognard J and Newton AC. PHLiPPing the switch on Akt and protein kinase C signaling. Trends Endocrinol. Metab. 2008; 19:223-30.

• Background Reference 02:

  Gao T, Furnari F and Newton AC. PHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth. Mol. Cell 2005; 18:13-24.

• Background Reference 03:

  Brognard J, Sierecki E, Gao T, et al. PHLPP and a second isoform, PHLPP2, differentially attenuate the amplitude of Akt signaling by regulating distinct Akt isoforms. Mol. Cell 2007; 25:917-31

• Background Reference 04:

  Gao MH, Miyanohara A, Feramisco JR, et al. Activation of PH-domain leucine-rich protein phosphatase 2 (PHLPP2) by agonist stimulation in cardiac myocytes expressing adenylyl cyclase type 6. Biochem. Biophys. Res. Commun. 2009; 384:193-8.

• Other Product Names:

  PHLPP2 Antibody: PHLPPL, KIAA0931, PHLPPL, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like, PHLPP-like

• Tested Applications:

  ELISA, WB, ICC, IF

• Protein ID:

  65301141

• Physical Properties:

  Liquid