PRC1 Antibody

• Background:

  PRC1 Antibody: PRC1 (Protein Regulator of Cytokinesis 1) is a key regulator of cytokinesis and has been identified as a substrate for several cyclin-dependent kinases (CDKs) . High levels of PRC1 expression occurs during S and G2/M and drops dramatically after mitosis exit and G1 entry. It is located in the nucleus during interphase and binds to the midzone of mitotic spindles during anaphase and is localized to the cell midbody during cytokinesis. Depletion of PRC1 has been shown to prevent cellular cleavage, but it has no effect on nuclear division, demonstrating the importance of PRC1 in mitosis.

• NCBI Gene ID:

  9055

• Swiss Prot:

  O43663

• Accession Number:

  NP_003972

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  PRC1 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate

• Concentration:

  1 mg/mL

• Buffer:

  PRC1 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PRC1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  At least three alternatively spliced transcript variants encoding distinct isoforms have been observed.

• Symbol:

  PRC1

• NCBI Official Name:

  Protein regulator of cytokinesis 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Jiang W, Jimenez G, Wells NJ, et al. PRC1: a human mitotic spindle-associated Cdk substrate protein required for cytokinesis. Mol. Cell 1998; 2:877-85.

• Background Reference 02:

  Pellman D, Bagget M, Tu H, et al. Two microtubule-associated proteins required for anaphase spindle movement in Saccharomyces Cerevisiae. J. Cell Biol. 1995; 130:1373-85.

• Background Reference 03:

  Kurasawa Y, Earnshaw WC, Mochizuki Y, et al. Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation. EMBO J. 2004; 23:3237-48.

• Background Reference 04:

  Mollinari C, Kleman JP, Jiang W, et al. PRC1 is a microtubule binding and bundling protein essential to maintain the mitotic spindle midzone. J. Cell Biol. 2002; 157:1175-86.

• Other Product Names:

  PRC1 Antibody: ASE1, Protein regulator of cytokinesis 1

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  4506039

• Physical Properties:

  Liquid