DDA Antibody

CAT: 0223-6483-01Size: 0.02 mgDry Ice: NoHazardous: No
CAT#:0223-6483-01Size:0.02 mg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
DDA Antibody: DDA1 (DET1 and DDB1 associated 1), along with DET1 and DDB1 and a member of the UBE2E group of canonical ubiquitin-conjugating enzymes, comprise DDD-E2 complexes, which interact with multiple ubiquitin E3 ligases. One of these E3 ligases is Cul4-containing E3 ligase complex CRL4. Cells depleted of DDA1 spontaneously accumulate double-stranded DNA breaks in a similar fashion as Cul4A-, Cul4B-, or WDR23-depleted cells, suggesting that DDA1 interacts with the CRL4 complex and may be involved in the ubiquitination and subsequent proteasomal degradation of target proteins.
NCBI Gene ID
79016
Swiss Prot
Q9BW61
Accession Number
NP_003253
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Homeostasis
Purification
DDA Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1401 - Mouse Heart Tissue Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (84%)
Buffer
DDA Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
DDA antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Symbol
DDA1
NCBI Official Name
DET1 and DDB1 associated 1
NCBI Organism
Homo sapiens
Background Reference 01
Pick E, Lau O, Tsuge T, et al. Mammalian DET1 regulates Cul4A activity and forms stable complexes with E2 ubiquitin-conjugating enzymes. Mol. Cell. Biol. 2007; 27:4708-19.
Background Reference 02
Olma MH, Roy M, Le Bihan T, et al. An interaction network of the mammalian COP9 signalsome identifies Dda1 as a core subunit of multiple Cul4-based E3 ligases. J. Cell Sci. 2009; 122:1035-44
Other Product Names
DDA Antibody: PCIA1, C19orf58, PCIA1, DET1- and DDB1-associated protein 1, Placenta cross-immune reaction antigen 1, PCIA-1
Tested Applications
ELISA, WB, IHC-P, IF
Protein ID
13129016
Physical Properties
Liquid

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