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SPT2 Antibody

CAT: 0223-6305-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-6305-01Size:0.02 mg
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Background
SPT2 Antibody: Serine palmitoyltransferase (SPT), which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPT2 is the long chain base subunit 2 of mammalian serine palmitoyltransferase. SPT2 is catalytically active but needs its related protein SPT1 for its stabilization and anchoring the holoenzyme to the cytosolic face of the endoplasmic reticulum. As in the case with SPT1, mutations in the SPT2 gene can cause hereditary sensory and autonomic neuropathy type I (HSAN-I), resulting from a loss of SPT activity as well as the accumulation of the atypical and neurotoxic sphingoid metabolite 1-deoxy-sphinganine.
NCBI Gene ID
9517
Swiss Prot
O15270
Accession Number
NP_004854
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
6305
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation in Cell lines (Figure 1) shows similar SPT2 expression profile in human cell lines detected by two independent anti-SPT2 antibodies that recognize different epitopes, 6305 against C-terminus domain and 64-238 against central domain. SPT2 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines6305 64-238
Field of Research
Homeostasis
Purification
SPT2 Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Rat (100%) .
Buffer
SPT2 Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
SPT2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Specificity
SPT2 antibody is predicted to not cross-react with SPT1.
Isoform
Human SPT2 has one isoform (562aa, 63kD) . Mouse SPT2 has one isoform (560aa, 63kD) and Rat SPT2 also has one isoform (560aa, 63kD) . 6305 can detect human, mouse and rat.
Symbol
SPTLC2
Positive Control 2
Cat. No. 17-201 - 3T3/BALB Cell Slide
Positive Control 3
Cat. No. 1204 - K562 Cell Lysate
Positive Control 4
Cat. No. 1219 - MCF7 Cell Lysate
Positive Control 5
Cat. No. 1220 - SK-N-SH Cell Lysate
NCBI Official Name
Serine palmitoyltransferase, long chain base subunit 2
NCBI Organism
Homo sapiens
Background Reference 01
Batheja et al. J. Histochem. & Cytochem. 2003; 51:687-96.
Background Reference 02
Nagiec et al. Gene 1996; 177:237-41
Background Reference 03
Yasuda et al. J. Biol. Chem. 2003; 278:4176-83.
Background Reference 04
Rotthier et al. Am. J. Hum. Genet. 2010; 87:513-22.
Other Product Names
SPT2 Antibody: LCB2, SPT2, HSN1C, LCB2A, NSAN1C, hLCB2a, KIAA0526, LCB2, Serine palmitoyltransferase 2, Long chain base biosynthesis protein 2, LCB 2
Tested Applications
ELISA, WB, ICC, IF
Protein ID
4758668
Physical Properties
Liquid

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