KLOTHO Antibody

• Background:

  KLOTHO Antibody: KLOTHO is the systemic anti-aging hormone within the glycosidase1 superfamily. It encodes a type I membrane protein that is abundant in the kidney and brain. In mice, a deficiency in KLOTHO expression leads to various systemic phenotypes resembling human aging such as arteriosclerosis, osteoporosis, and skin atrophy together with growth retardation, short life-span and infertility. Transgenic mice overexpressing KLOTHO have an extended life span by inhibiting insulin/IGF1 signaling. KLOTHO is involved in the regulation of calcium/phosphorus homeostasis by inhibiting the synthesis of active vitamin D and identified as a potential tumor suppressor.

• NCBI Gene ID:

  9365

• Swiss Prot:

  Q9UEF7

• Accession Number:

  NP_004786

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  6107

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation in Cell lines (Figure 1) shows similar KLOTHO expression profile in human and mouse cell lines detected by two independent anti-KLOTHO antibodies that recognize different epitopes, 6107 against central domain and competitor antibody against human recombinant protein. KLOTHO proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines6107 competitor antibody Recombinant Protein Test (Figure 2) : Anti-KLOTHO antibodies (6107) detected human KLOTHO recombinant protein at different concentrations. Recombinant Protein Test6107

• Field of Research:

  Signal Transduction

• Purification:

  KLOTHO Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1203 - A549 Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  KLOTHO Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  KLOTHO antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  Three isoforms of KLOTHO are known to exist.

• Isoform:

  Human KLOTHO has 2 isoforms, including isoform 1 (1012aa, 116kD) and isoform 2 (549aa, 62kD) . Mouse KLOTHO has 2 isoforms, including isoform 1 (1014aa, 116kD) and isoform 2 (550aa, 62kD) . Rat KLOTHO has one isoform (1014aa, 117kD) . 6107 can detect human, mouse and rat.

• Symbol:

  KL

• Positive Control 2:

  Cat. No. 1204 - K562 Cell Lysate

• Positive Control 3:

  Cat. No. 1205 - Jurkat Cell Lysate

• Positive Control 4:

  Cat. No. 1282 - 3T3/NIH Cell Lysate

• NCBI Official Name:

  Klotho

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Kuro-o et al. Nature1997; 390:45-51.

• Background Reference 02:

  Kurosu et al. Science2005; 309:1829-33.

• Background Reference 03:

  Liu et al. Science 2007; 317:803-6.

• Background Reference 04:

  Wolf et al. Oncogene2008; 27:7094-105.

• Other Product Names:

  KLOTHO Antibody: Klotho

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  24497614

• Physical Properties:

  Liquid