APC2 Antibody

• Background:

  APC2 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC2 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. Like APC11, APC2 contains cullin and RING finger domains that are thought to be important in regulating ubiquitination activity.

• NCBI Gene ID:

  10297

• Swiss Prot:

  O95996

• Accession Number:

  BAA34611

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  APC2 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  APC2 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  APC2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  APC2

• Positive Control 2:

  Cat. No. 17-001 - HeLa Cell Slide

• NCBI Official Name:

  Adenomatosis polyposis coli 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56.

• Background Reference 02:

  Jorgensen PM, Graslund S, Betz R, et al. Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex. Gene2001; 262:51-9.

• Background Reference 03:

  Yu H, Peters JM, King RW, et al. Identification of a cullin homology region in a subunit of the anaphase-promoting complex. Science1998; 279:1219-22.

• Background Reference 04:

  Zacharie W, Shevchenko A, Andrews PD, et al. Mass spectrometric analysis of the anaphase-promoting complex from yeast: identification of a subunit related to cullins. Science1998; 1216-19.

• Other Product Names:

  APC2 Antibody: APCL, APCL, Adenomatous polyposis coli protein 2, Adenomatous polyposis coli protein-like, APC-like

• Tested Applications:

  ELISA, WB, ICC, IF

• Protein ID:

  3894265

• Physical Properties:

  Liquid