Maelstrom Antibody

• Background:

  Maelstrom Antibody: The mammalian homolog of the Drosophila protein Maelstrom is expressed in the male germline and localizes to the sex body in spermatocytes and the chromatoid body in round spermatids. Similar to its expression in Drosophila, Maelstrom is a component of nuages, a germ-cell specific organelle and is thought to be essential for spermatogenesis and transposon repression during meiosis. In humans, Maelstrom has been found to be expressed only in the testis and in various cancer cell lines. Treatment of these cell lines with the demethylating agent 5'-Aza-2-Deoxycytidine significantly upregulated Maelstrom levels, indicating that its expression is regulated by DNA methylation.

• NCBI Gene ID:

  84944

• Swiss Prot:

  Q96JY0

• Accession Number:

  AAY82463

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  Maelstrom Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (94%), Pig: (88%)

• Buffer:

  Maelstrom Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Maelstrom antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  MAEL

• Positive Control 2:

  Cat. No. 17-001 - HeLa Cell Slide

• NCBI Official Name:

  Maelstrom homolog (Drosophila)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Clegg NJ, Frost DM, Larkin MK, et al. Maelstrom is required for an early step in the establishment of Drosophila oocyte polarity: posterior localization of grk mRNA. Development1997; 124:4661-71.

• Background Reference 02:

  Costa Y, Speed RM, Gautier P, et al. Mouse MAELSTROM: the link between meiotic silencing of unsynapsed chromatin and microRNA pathway? Hum. Mol. Genet.2006; 15:2324-34.

• Background Reference 03:

  Soper SFC, van der Heijen GW, Hardiman TC, et al. Mouse Maelstrom, a component of nuage, is essential for spermatogenesis and transposon repression in meiosis. Dev. Cell2008; 15:285-97.

• Background Reference 04:

  Xiao L, Wang Y, Zhou Y, et al. Identification of a novel human/testis gene MAEL that is regulated by DNA methylation. Mol. Biol. Rep.2009; epub.

• Other Product Names:

  Maelstrom Antibody: CT128, SPATA35, Protein maelstrom homolog

• Tested Applications:

  ELISA, WB, ICC, IF

• Protein ID:

  67866977

• Physical Properties:

  Liquid