Beta-actin Antibody

• Background:

  Beta-actin Antibody: Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

• NCBI Gene ID:

  60

• Swiss Prot:

  P60709

• Accession Number:

  AAH02409

• Host:

  Chicken

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  Beta-actin Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1201 - HeLa Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Sheep: (100%), Rabbit: (100%), Pig: (100%), Horse: (100%), Chicken: (100%), Guinea pig: (100%), Bovine: (100%)

• Buffer:

  Beta-actin Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Beta-actin antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgY

• Symbol:

  ACTB

• NCBI Official Name:

  Actin, beta

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lambrechts A, Van Troys, M and Ampe C. The actin cytoskeleton in normal and pathological cell motility. Int. J. Biochem. Cell Biol.2004; 36:1890-909.

• Background Reference 02:

  Gourlay CW and Ayscough KR. The actin cytoskeleton: a key regulator of apoptosis and ageing? Nat. Rev.2005; 6:583-9.

• Other Product Names:

  Beta-actin Antibody: BRWS1, PS1TP5BP1, Actin, cytoplasmic 1, Beta-actin

• Tested Applications:

  ELISA, WB

• Protein ID:

  12803203

• Physical Properties:

  Liquid