MDA5 Antibody

• Background:

  MDA5 Antibody: The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-κ B. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C) . MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.

• NCBI Gene ID:

  64135

• Swiss Prot:

  Q9BYX4

• Accession Number:

  NP_071451

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Innate Immunity

• Purification:

  MDA5 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1224 - Daudi Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  MDA5 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  MDA5 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  IFIH1

• Positive Control 2:

  Cat. No. 1369 - Human Lymph node Tissue Lysate

• Positive Control 3:

  Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide

• NCBI Official Name:

  Interferon induced with helicase C domain 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Akira S, Uematsu S, and Takeuchi O. Pathogen recognition and innate immunity. Cell 2006; 124:783-801.

• Background Reference 02:

  Hiscott J, Nguyen T-LA, Arguello M, et al. Manipulation of the nuclear factor-kappaB pathway and the innate immune response by viruses. Oncogene 2006; 25:6844-67.

• Background Reference 03:

  Kang D, Gopalrishnan RV, Lin L, et al. Expression analysis and genomic characterization of human melanoma differentiation associated gene-5, mda-5: a novel type I interferon-responsive apoptosis-inducing gene. Oncogene 2004; 23:1789-800.

• Background Reference 04:

  Andrejeva J, Childs KS, Young DF, et al. The V proteins of the paramyxoviruses bind the IFN-inducible RNA helicase, mda-5, and inhibit its activation of the IFN-beta promoter. Proc. Natl. Acad. Sci. USA 2004; 101:17264-9.

• Other Product Names:

  MDA5 Antibody: Hlcd, MDA5, MDA-5, RLR-2, IDDM19, RH116, Interferon-induced helicase C domain-containing protein 1, Clinically amyopathic dermatomyositis autoantigen 140 kDa, CADM-140 autoantigen

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  27886568

• Physical Properties:

  Liquid