Sumo Antibody

• Background:

  Sumo Antibody: The sumo family of proteins is related both structurally and functionally to ubiquitin in that they are post-translationally attached to the e-amino group of a lysine residue of the substrate protein. This sumoylation plays a number of roles in DNA replication and repair, protein targeting to various subnuclear structures, and the regulation of numerous cellular processes including the inflammatory response in mammalian cells. Sumo was initially identified as a covalent modification of RanGAP1 in studies on nuclear import in mammalian cells. More recently, sumo has been shown to be involved in the regulation of transcription factors, possibly by enhancing their interactions with co-repressors. Sumo is also thought to play some role in the modulation of ubiquitin-mediated degradation of proteins by acting as an inhibitor. At least four different isoforms of sumo are known to exist; Sumo antibody will only recognize isoform 1.

• NCBI Gene ID:

  7341

• Swiss Prot:

  P63165

• Accession Number:

  AAH66306

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  Sumo Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1209 - HL60 Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Pig: (100%), Bovine: (100%), Chicken: (93%)

• Buffer:

  Sumo Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Sumo antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  SUMO1

• Positive Control 2:

  Cat. No. 17-009 - HL-60 Cell Slide

• NCBI Official Name:

  SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Dohmen RJ. Sumo protein modification. Biochim. Biophys. Acta 2004; 1695:113-31.

• Background Reference 02:

  Matunis MJ, Coutavas E, and Blobel G. A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex. J. Cell Biol. 1996; 135:1457-70.

• Background Reference 03:

  Gill G. Something about SUMO inhibits transcription. Curr. Opin. Genet. Dev. 2005; 15:536-41.

• Background Reference 04:

  Desterro JM, Rodriguez MS, and Hay RT. SUMO-1 modification of IkappaBalpha inhibits NFkappaB activation. Mol. Cell 1998; 2:233-9.

• Other Product Names:

  Sumo Antibody: DAP1, GMP1, PIC1, SMT3, UBL1, OFC10, SENP2, SMT3C, SMT3H3, OK/SW-cl.43, Small ubiquitin-related modifier 1, GAP-modifying protein 1, SUMO-1

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  42490984

• Physical Properties:

  Liquid