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PKR Antibody

CAT: 0223-3949-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-3949-01Size:0.02 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
PKR Antibody: The interferon-inducible, double-stranded RNA (dsRNA) -dependent protein kinase PKR is a member of the eukaryotic initiation factor-2 alpha (eIF2-alpha) kinase family, possessing serine-threonine kinase activity and two dsRNA-binding motifs that acts as part of the innate immune system. Upon binding dsRNA, PKR undergoes a conformational change leading to its activation and its phosphorylation of the translation factor eIF2, resulting in a general shutdown of protein synthesis and induction of apoptosis through upregulation of caspase-8 and capsase-9 activity in order to prevent the production of more viruses. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.
NCBI Gene ID
5610
Swiss Prot
P19525
Accession Number
NP_002750
Host
Rabbit
Reactivity
Human, Rat
Immunogen
3949
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation in Cell lines (Figure 2) shows similar PKR expression profile in human and mouse cell lines detected by two independent anti-PKR antibodies that recognize different epitopes, 3947 against C-terminus domain and 3949 against N-terminus domain. PKR proteins are detected in all the tested human cell lines except SK-N-SH cell line at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3949
Field of Research
Innate Immunity
Purification
PKR Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1208 - THP-1 cell lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Mouse (86%)
Buffer
PKR Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
PKR antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Isoform
Human PKR has 2 isoforms, including isoform 1 (551aa, 62.1kD) and isoform S (510aa, 57.4kD. This antibody detects both human isoforms. Mouse PKR has 1 isoform (515aa, 58.3kD) . Rat PKR has only one isoform identified so far (513aa, 58.3kD) .
Symbol
EIF2AK2
Positive Control 2
Cat. No. 1462 - Rat Lung Tissue Lysate
Positive Control 3
Cat. No. 1224 - Daudi Cell Lysate
Positive Control 4
Cat. No. 1202– A431 Cell Lysate
NCBI Official Name
Eukaryotic translation initiation factor 2-alpha kinase 2
NCBI Organism
Homo sapiens
Background Reference 01
Meurs et al. Cell 1990; 62:379-90.
Background Reference 02
Saelens et al. J. Biol. Chem. 2001; 276:41620-8.
Background Reference 03
Gil and Esteban. Apoptosis 2000; 5:107-14.
Background Reference 04
Gil et al. FEBS Lett. 2002; 19:3665-74.
Other Product Names
PKR Antibody: PKR, PRKR, EIF2AK1, PKR, Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2
Tested Applications
ELISA, WB, IHC-P
Protein ID
31747519
Physical Properties
Liquid

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