ATF6 Antibody

• Background :

  ATF6 Antibody: Disruptions of protein folding and maturation in the endoplasmic reticulum (ER) result in the activation of the unfolded protein response (UPR), an integrated cellular signaling pathway that transmits information from the ER lumen to the cytoplasm and nucleus. Activating transcription factor 6 (ATF6) as well as the ER-transmembrane protein kinases IRE1p and PERK are the major transducers of the UPR. ATF6 is an ER transmembrane protein that is normally bound to the ER chaperone GRP78, but upon ER stress is released from GRP78 and proteolytically cleaved to yield a cytosolic fragment which then migrates to the nucleus, and together with the transcription factor XBP-1, activates transcription of UPR-responsive genes. ATF6 has two isoforms (ATF6α and ATF6β ) ; only ATF6α is recognized by this antibody.

• NCBI Gene ID :

  22926

• Swiss Prot :

  P18850

• Accession Number :

  NP_031374

• Host :

  Rabbit

• Reactivity :

  Human, Mouse

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Signal Transduction, Apoptosis

• Purification :

  ATF6 Antibody is affinity chromatography purified via peptide column.

• Positive Control :

  Cat. No. 1287 - EL4 Cell Lysate

• Concentration :

  1 mg/mL

• Homology :

  Predicted species reactivity based on immunogen sequence: Rat: (100%)

• Buffer :

  ATF6 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  ATF6 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment :

  IgG

• Symbol :

  ATF6

• Positive Control 2 :

  Cat. No. 17-012 - MCF7 Cell Slide

• NCBI Official Name :

  Activating transcription factor 6

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Liu CY and Kaufman RJ. The unfolded protein response. J. Cell Sci. 2003; 1861-2.

• Background Reference 02 :

  Haze K, Yoshida H, Yanagi H, et al. Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic stress. Mol. Cell. Biol. 1999; 10:3787-99.

• Background Reference 03 :

  Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94) : functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.

• Background Reference 04 :

  Yoshida H, Matsui T, Yamamoto T, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1p in response to ER stress to produce a highly active transcription factor. Cell 2001; 107:881-91.

• Other Product Names :

  ATF6 Antibody: ATF6A, Cyclic AMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, cAMP-dependent transcription factor ATF-6 alpha

• Tested Applications :

  ELISA, WB, IHC-P, ICC, IF

• Protein ID :

  56786157

• Physical Properties :

  Liquid