ATF4 Antibody

• Background:

  ATF4s a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2) . The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

• NCBI Gene ID:

  468

• Swiss Prot:

  P18848

• Accession Number:

  NP_001666

• Host:

  Rabbit

• Reactivity:

  Human, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATF4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ATF4 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  39 kDa, 39 kDa

• Applications Notes:

  ATF4 antibody can be used for detection of ATF4 by ELISA at 1:62500. ATF4 antibody can be used for detection of ATF4 by western blot at 5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  ATF4

• NCBI Official Name:

  Activating transcription factor 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lassot, I., et al., (2005) J. Biol. Chem. 280 (50), 41537-41545.

• Other Product Names:

  ATF4, CREB2, TXREB, CREB-2, TAXREB67

• Tested Applications:

  ELISA, WB

• Protein ID:

  33469976

• Physical Properties:

  Liquid