PHAP I Antibody

• Background:

  PHAP I Antibody: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.

• NCBI Gene ID:

  8125

• Swiss Prot:

  P39687

• Accession Number:

  P39687

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  3145

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation in Cell lines (Figure 2) shows similar PHAP I expression profile in human, mouse and rat cell lines detected by two independent anti-PHAP I antibodies that recognize different epitopes, 3145 against C-terminus domain and 3151 against C-terminus different domain. PHAP I proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3145 3151 KD validation (Figure 6) : Anti-PHAP I antibody (3151) specificity was further verified by PHAP I specific knockdown. PHAP I signal in T47D cells transfected with PHAP I siRNAs was disrupted in comparison with that in cells transfected with control siRNAs. KD validation3151 Regulated expression validation (Figure 7, 8, 9) : PHAP I expression detected by anit-PHAP I antibodies (3151) was up-regulated in breast cancer tissues of patients (Figure 7) and the breast cancer cell line (Figure 8) as well as in the Atxn1 KO mice (Figure 9) . Regulated expression validation3151

• Field of Research:

  Apoptosis

• Purification:

  PHAP I Antibody is DEAE purified.

• Positive Control:

  Cat. No. 1207 - Raji Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  PHAP I Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PHAP I antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  This polyclonal antibody has no cross-reaction to PHAP I2a and PHAP III.

• Isoform:

  Human PHAP I has one isoform (249aa, 28.6kD) . Mouse PHAP I has one isoform (247aa, 28.5kD) and rat PHAP I also has one isoform (247aa, 28.6kD) . 3151 can detect all isoforms of human, mouse and rat.

• Symbol:

  ANP32A

• Positive Control 2:

  Cat. No. 17-007 - Raji Cell Slide

• NCBI Official Name:

  Acidic (leucine-rich) nuclear phosphoprotein 32 family, member A

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Jiang et al. Science. 2003;299 (5604) :223-6.

• Background Reference 02:

  Nicholson and Thornberry. Science. 2003 10;299 (5604) :214-5.

• Citation 01:

  16489023

• Citation 02:

  23630944

• Citation 03:

  23652204

• Citation 04:

  Parrish. Regulation of Apoptosis Following Mitochondrial Cytochrome c Release. Department of Pharmacology and Molecular Cancer Biology, Duke University. PhD Thesis, 2010.

• Other Product Names:

  PHAP I Antibody: LANP, MAPM, PP32, HPPCn, PHAP1, PHAPI, I1PP2A, C15orf1, LANP, Acidic leucine-rich nuclear phosphoprotein 32 family member A, Acidic nuclear phosphoprotein pp32

• Tested Applications:

  ELISA, WB, ICC, IF

• Protein ID:

  730318

• Physical Properties:

  Liquid