EndoG Antibody

• Background:

  EndoG Antibody: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndooG also cleaves DNA in vitro.

• NCBI Gene ID:

  2021

• Swiss Prot:

  Q14249

• Accession Number:

  NP_004426

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  3035

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  KD validation (Figure 13, 14) : Anti-EndoG antibody (3035) specificity was further verified by EndoG specific knockdown. EndoG signal in human cancer cell lines transfected with EndoG siRNAs was disrupted in comparison with that in cells transfected with control siRNAs. KD validation3035 Localization validation (Figure 7, 8, 10, 11) : EndoG translocation was observed in different cell lines and tissues after treatment. From mitochondria to cytoplasm: figure 7 and figure 8. From cytosol to nucleus: figure 10 and figure 11. Localization validation

• Field of Research:

  Apoptosis, Cancer

• Purification:

  EndoG Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  EndoG Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  EndoG antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Human EndoG has only one isoform (297aa, 33kD) . Mouse EndoG has one isoform (294aa, 32kD) and Rat EndoG also has one isoform (294aa, 32kD) . 3035 can detect human, mouse and rat isoform.

• Symbol:

  ENDOG

• Positive Control 2:

  Cat. No. 1212 - 3T3 Cell Lysate

• Positive Control 3:

  Cat. No. 1307 - Human Pancreas Tissue Lysate

• Positive Control 4:

  Cat. No. 11-101 - Human Pancreas Tissue Slide

• NCBI Official Name:

  Endonuclease G

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Li et al. Nature 2001;412 (6842) :95-9.

• Background Reference 02:

  Parrish et al. Nature 2001;412 (6842) :90-4.

• Background Reference 03:

  Hengartner. Nature. 2001;412 (6842) :27, 29.

• Background Reference 04:

  Widlak et al. J Biol Chem. 2001;276 (51) :48404-9.

• Citation 01:

  18504456

• Citation 02:

  16151635

• Citation 03:

  17292393

• Citation 04:

  16689664

• Citation 05:

  20574921

• Citation 06:

  17955488

• Citation 07:

  19459857

• Citation 08:

  19808980

• Citation 09:

  16728972

• Citation 10:

  16046141

• Citation 11:

  17050806

• Citation 12:

  25865938

• Citation 13:

  18699742

• Citation 14:

  19793916

• Citation 15:

  21377520

• Citation 16:

  28122282

• Other Product Names:

  EndoG Antibody: Endonuclease G, mitochondrial, Endo G

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  53759134

• Physical Properties:

  Liquid