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EndoG Antibody

CAT: 0223-3035-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-3035-01Size:0.02 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
EndoG Antibody: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndooG also cleaves DNA in vitro.
NCBI Gene ID
2021
Swiss Prot
Q14249
Accession Number
NP_004426
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
3035
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
KD validation (Figure 13, 14) : Anti-EndoG antibody (3035) specificity was further verified by EndoG specific knockdown. EndoG signal in human cancer cell lines transfected with EndoG siRNAs was disrupted in comparison with that in cells transfected with control siRNAs. KD validation3035 Localization validation (Figure 7, 8, 10, 11) : EndoG translocation was observed in different cell lines and tissues after treatment. From mitochondria to cytoplasm: figure 7 and figure 8. From cytosol to nucleus: figure 10 and figure 11. Localization validation
Field of Research
Apoptosis, Cancer
Purification
EndoG Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
1 mg/mL
Buffer
EndoG Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
EndoG antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Isoform
Human EndoG has only one isoform (297aa, 33kD) . Mouse EndoG has one isoform (294aa, 32kD) and Rat EndoG also has one isoform (294aa, 32kD) . 3035 can detect human, mouse and rat isoform.
Symbol
ENDOG
Positive Control 2
Cat. No. 1212 - 3T3 Cell Lysate
Positive Control 3
Cat. No. 1307 - Human Pancreas Tissue Lysate
Positive Control 4
Cat. No. 11-101 - Human Pancreas Tissue Slide
NCBI Official Name
Endonuclease G
NCBI Organism
Homo sapiens
Background Reference 01
Li et al. Nature 2001;412 (6842) :95-9.
Background Reference 02
Parrish et al. Nature 2001;412 (6842) :90-4.
Background Reference 03
Hengartner. Nature. 2001;412 (6842) :27, 29.
Background Reference 04
Widlak et al. J Biol Chem. 2001;276 (51) :48404-9.
Citation 01
18504456
Citation 02
16151635
Citation 03
17292393
Citation 04
16689664
Citation 05
20574921
Citation 06
17955488
Citation 07
19459857
Citation 08
19808980
Citation 09
16728972
Citation 10
16046141
Citation 11
17050806
Citation 12
25865938
Citation 13
18699742
Citation 14
19793916
Citation 15
21377520
Citation 16
28122282
Other Product Names
EndoG Antibody: Endonuclease G, mitochondrial, Endo G
Tested Applications
ELISA, WB, IHC-P, IF
Protein ID
53759134
Physical Properties
Liquid

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