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Smac Antibody

CAT: 0223-2411-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-2411-01Size:0.02 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Smac Antibody: The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO. Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.
NCBI Gene ID
66593
Swiss Prot
Q9JIQ3
Accession Number
AF203914
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
2411
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation in Cell lines (Figure 2) shows similar Smac expression profile in human and mouse cell lines detected by two independent anti-Smac antibodies that recognize different epitopes, 2409 against human C-terminus domain and 2411 against murine C-terminus domain. Smac proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines2409 2411 KO Validation (Figure 6) : Anti-Smac antibody specificity was further verified by Smac knockout mice. Smac signal smac KO mice was disrupted as compared to control. KO Validation Induced Expression Validation (Figure 6,9) : Smac expression detected by anit-Smac antibodies was up-regulated by STS or rPVL treatment. Induced Expression Validation Overexpression Validation (Figure 10) : Smac overexpression was detected by anit-Smac antibodies on smac transfected HEK293T cells. Overexpression Validation
Field of Research
Apoptosis, Cancer
Purification
Smac Antibody is DEAE purified.
Positive Control
Cat. No. 1401 - Mouse Heart Tissue Lysate
Concentration
1 mg/ml
Buffer
Smac Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Smac antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Isoform
Human Smac has 3 isoforms, including isoform 1 (239aa, 27.1kD), isoform 2 (186aa, 21.2kD), and isoform 3 (195aa, 22.3kD) . Mouse Smac has one isoform (237aa, 26.8kD) and Rat Smac also has one isoform (237aa, 26.9kD) . 2411 can detect human, mouse and rat.
Symbol
Diablo
NCBI Official Name
Diablo homolog (Drosophila)
NCBI Organism
Mus musculus
Background Reference 01
Du et al. Cell 2000; 102:33-42.
Background Reference 02
Verhagen et al. Cell 2000;102:43-53.
Background Reference 03
Srinivasula et al. J. Biol. Chem. 2000; 275:36152-7.
Background Reference 04
Chai et al. Nature 2000; 406:855-62.
Citation 01
17562856
Citation 02
21947931
Citation 03
15784731
Citation 04
16276417
Citation 05
22413863
Citation 06
15489959
Citation 07
21397847
Citation 08
18339863
Citation 09
26903540
Citation 10
18259190
Citation 11
18084240
Citation 12
18202705
Citation 13
16167065
Citation 14
20085492
Citation 15
24305822
Citation 16
19492850
Citation 17
17412759
Citation 18
18298651
Citation 19
17167046
Citation 20
15328340
Other Product Names
Smac Antibody: Smac, AU040403, 0610041G12Rik, 1700006L01Rik, Smac, Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI
Tested Applications
ELISA, WB, IHC-P, IF, IP
Protein ID
8953908
Physical Properties
Liquid

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