IKAP Antibody

• Background:

  IKAP Antibody: IKAP was initially identified as a scaffold protein of the Iκ B kinase complex that could bind to IKKα , IKKβ , NF-κ B, and the NF-κ B-inducing kinase (NIK), although later evidence has cast doubt on this. More recent reports show that mutations in IKAP such as a frameshift leading to a truncated protein or a missense mutation that leads to defective phosphorylation are responsible for the autosomal recessive genetic disease familial dysautonomia (FD) . Reports indicating that it forms part of the RNA polymerase II transcription elongation complex suggest that this disease may be due to compromised transcription elongation. More recently, it was shown that IKAP associates with c-Jun N-terminal kinase (JNK) and could specifically enhance JNK activation induced by the upstream JNK activators MEKK1 and ASK1, indicating another possible cause for FD.

• NCBI Gene ID:

  8518

• Swiss Prot:

  O95163

• Accession Number:

  AAC64258

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  IKAP Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1288 - A20 Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Rabbit: (88%), Rat: (75%)

• Buffer:

  IKAP Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  IKAP antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  At least two isoforms of IKAP are known two exist, this antibody will detect both isoforms.

• Symbol:

  IKBKAP

• Positive Control 2:

  Cat. No. 17-208 - A-20 Cell Slide

• NCBI Official Name:

  Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Cohen L, Henzel WJ, and Baeuerle PA. IKAP is a scaffold protein of the IkB kinase complex. Nature 1998; 395:292-6.

• Background Reference 02:

  Krappmann D, Hatada EN, Tegethoff S, et al. The I kappa B kinase (IKK) complex is tripartite and contains IKK gamma but not IKAP as a regular component. J. Biol. Chem. 2000; 275:29779-87.

• Background Reference 03:

  Anderson SL, Coli R, Daly IW, et al. Familial dysautonomia is caused by mutations of the IKAP gene. Am. J. Hum. Genet. 2001; 68:753-8.

• Background Reference 04:

  Hawkes NA, Otero G, Winkler GS, et al. Purification and characterization of the human elongator complex. J. Biol. Chem. 2002; 277:3047-52.

• Other Product Names:

  IKAP Antibody: FD, DYS, ELP1, IKAP, IKI3, TOT1, Elongator complex protein 1, IkappaB kinase complex-associated protein

• Tested Applications:

  ELISA, WB, ICC, IF

• Protein ID:

  3757822

• Physical Properties:

  Liquid