RIP3 Antibody

CAT: 0223-2283-01Size: 0.02 mgDry Ice: NoHazardous: No
CAT#:0223-2283-01Size:0.02 mg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
RIP3 Antibody: Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-κ B activation. Overexpression of RIP3 induces apoptosis and NF-κ B activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.
NCBI Gene ID
56532
Swiss Prot
Q9QZL0
Accession Number
AAF03133
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
2283
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
KO Validation (Figure 7,9,13) shows RIP3 expression detected by anti-RIP3 antibodies (2283) was disrupted in multiple tissues of RIP3 KO mice (figure 7, 13) and in RIP3 KO MEF cells (figure 9) . KO Validation KD validation (Figure 10) : Anti-RIP3 antibody (2283) specificity was further verified by RIP3 specific knockdown. RIP3 signal in L929 cells transfected with RIP3 shRNA was disrupted in comparison with that in cells transfected with control shRNA. KD validation2283 Regulated expression validation (Figure 7,11,13) : RIP3 expression detected by anit-RIP3 antibodies (2283) was up-regulated by treatment of cerulein (figure 7), LPS (figure 11) or acetaminophen (figure 13) in different tissues or cells. Regulated expression validation2283 Overexpression validation (Figure 12) : RIP3 overexpression detected by anit-RIP3 antibodies (2283) was observed in three cell lines stably transfected with RIP3. Overexpression validation2283
Field of Research
Apoptosis
Purification
RIP3 Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1202 – A431 Cell Lysate
Concentration
1 mg/ml
Buffer
RIP3 Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
RIP3 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Isoform
Mouse RIP3 has one isoform (486aa, 53kD) . Human RIP3 has 3 isoforms, including isoform 1 (518aa, 57kD), isoform 2 (252aa, 28kD) and isoform 3 (231aa, 25kD) . Rat RIP3 also has one isoform (478aa, 52kD) . 2283 can detect can detect isoforms of mouse and rat as well as human isoform 1.
Symbol
Ripk3
Positive Control 2
Cat. No. 1282 - 3T3/NIH Cell Lysate
Positive Control 3
Cat. No. 1285- C2C12 Cell Lysate
Positive Control 4
Cat. No. 1212- 3T3/Balb Cell Lysate
NCBI Official Name
Receptor-interacting serine-threonine kinase 3
NCBI Organism
Mus musculus
Background Reference 01
Yu et al. Curr Biol. 1999;9 (10) :539-42.
Background Reference 02
Sun et al. J Biol Chem. 1999;274 (24) :16871-5.
Background Reference 03
Pazdernik et al. Mol Cell Bio. 1999; 19 (10) :6500-8 (WD0102)
Citation 01
19524512
Citation 02
21368761
Citation 03
23201684
Citation 04
26943325
Citation 05
28981102
Citation 06
23744808
Citation 07
22817896
Citation 08
24441827
Citation 09
25326752
Citation 10
25939025
Citation 11
24012422
Citation 12
27321907
Citation 13
28085133
Citation 14
26418225
Citation 15
23734789
Citation 16
23348743
Citation 17
22262768
Citation 18
28871172
Citation 19
27532042
Citation 20
21402742
Other Product Names
RIP3 Antibody: Rip3, AW107945, 2610528K09Rik, Rip3, RIP-like protein kinase 3, RIP-3
Tested Applications
ELISA, IF, IHC-P, WB, IP
Protein ID
6063101
Physical Properties
Liquid

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