RIP3 Antibody

• Background:

  RIP3 Antibody: Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3. RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-κ B activation. Overexpression of RIP3 induces apoptosis and NF-κ B activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues.

• NCBI Gene ID:

  56532

• Swiss Prot:

  Q9QZL0

• Accession Number:

  AAF03133

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  2283

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  KO Validation (Figure 7,9,13) shows RIP3 expression detected by anti-RIP3 antibodies (2283) was disrupted in multiple tissues of RIP3 KO mice (figure 7, 13) and in RIP3 KO MEF cells (figure 9) . KO Validation KD validation (Figure 10) : Anti-RIP3 antibody (2283) specificity was further verified by RIP3 specific knockdown. RIP3 signal in L929 cells transfected with RIP3 shRNA was disrupted in comparison with that in cells transfected with control shRNA. KD validation2283 Regulated expression validation (Figure 7,11,13) : RIP3 expression detected by anit-RIP3 antibodies (2283) was up-regulated by treatment of cerulein (figure 7), LPS (figure 11) or acetaminophen (figure 13) in different tissues or cells. Regulated expression validation2283 Overexpression validation (Figure 12) : RIP3 overexpression detected by anit-RIP3 antibodies (2283) was observed in three cell lines stably transfected with RIP3. Overexpression validation2283

• Field of Research:

  Apoptosis

• Purification:

  RIP3 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1202 – A431 Cell Lysate

• Concentration:

  1 mg/ml

• Buffer:

  RIP3 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  RIP3 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Mouse RIP3 has one isoform (486aa, 53kD) . Human RIP3 has 3 isoforms, including isoform 1 (518aa, 57kD), isoform 2 (252aa, 28kD) and isoform 3 (231aa, 25kD) . Rat RIP3 also has one isoform (478aa, 52kD) . 2283 can detect can detect isoforms of mouse and rat as well as human isoform 1.

• Symbol:

  Ripk3

• Positive Control 2:

  Cat. No. 1282 - 3T3/NIH Cell Lysate

• Positive Control 3:

  Cat. No. 1285- C2C12 Cell Lysate

• Positive Control 4:

  Cat. No. 1212- 3T3/Balb Cell Lysate

• NCBI Official Name:

  Receptor-interacting serine-threonine kinase 3

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Yu et al. Curr Biol. 1999;9 (10) :539-42.

• Background Reference 02:

  Sun et al. J Biol Chem. 1999;274 (24) :16871-5.

• Background Reference 03:

  Pazdernik et al. Mol Cell Bio. 1999; 19 (10) :6500-8 (WD0102)

• Citation 01:

  19524512

• Citation 02:

  21368761

• Citation 03:

  23201684

• Citation 04:

  26943325

• Citation 05:

  28981102

• Citation 06:

  23744808

• Citation 07:

  22817896

• Citation 08:

  24441827

• Citation 09:

  25326752

• Citation 10:

  25939025

• Citation 11:

  24012422

• Citation 12:

  27321907

• Citation 13:

  28085133

• Citation 14:

  26418225

• Citation 15:

  23734789

• Citation 16:

  23348743

• Citation 17:

  22262768

• Citation 18:

  28871172

• Citation 19:

  27532042

• Citation 20:

  21402742

• Other Product Names:

  RIP3 Antibody: Rip3, AW107945, 2610528K09Rik, Rip3, RIP-like protein kinase 3, RIP-3

• Tested Applications:

  ELISA, IF, IHC-P, WB, IP

• Protein ID:

  6063101

• Physical Properties:

  Liquid