Acinus Antibody

• Background:

  Acinus Antibody: Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE. A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus) . Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS'.

• NCBI Gene ID:

  22985

• Swiss Prot:

  Q9UKV3

• Accession Number:

  AAD56724

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis, Cancer

• Purification:

  Acinus Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1204 - K562 Cell Lysate

• Concentration:

  1 mg/ml

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse: (93%)

• Buffer:

  Acinus Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Acinus antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  220 kDa

• Fragment:

  IgG

• Symbol:

  ACIN1

• Positive Control 2:

  Cat. No. 17-004 - K-562 Cell Slide

• NCBI Official Name:

  Apoptotic chromatin condensation inducer 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999 ;401:127-8.

• Background Reference 02:

  Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y. Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Nature 1999 401:168-73.

• Other Product Names:

  Acinus Antibody: ACN, ACINUS, fSAP152, KIAA0670, Apoptotic chromatin condensation inducer in the nucleus, Acinus

• Tested Applications:

  ELISA, WB, ICC, IF

• Protein ID:

  5931959

• Physical Properties:

  Liquid