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IgG2b Protein, Mouse, Recombinant

CAT: 0882-PM53350M0-01Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0882-PM53350M0-01Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Overview
A DNA sequence encoding the Mouse IgG2b Fc region (P01867-2) (Glu 97-Lys 335) was expressed and purified.
Synonyms
IgG2b Protein, Mouse; IgG2b-Fc Protein, Mouse
UniProt
P01867-2
Expression System
HEK293 Cells
Tag
No tag
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 92 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
IgG Fc Control proteins
Molecular Weight
The recombinant mouse IgG2b-Fc consists of 239 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, the apparent molecular mass of IgG2b-Fc is approximately 37 kDa in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
Ighg2b
Species
Mouse
Uniprot URL
https://www.uniprot.org/uniprot/P01867-2
AA Sequence
Glu97-Lys335

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