CCL21 Protein, Human, Recombinant

Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokine ligand 21 (CCL21), also known as 6Ckine, exodus-2, and secondary lymphoid-tissue chemokine (SLC), is a small cytokine belonging to the C-C chemokine family. CCL21 takes its name 6Ckine for its consititutively six conserved cysteine residues but not four cysteines typical to chemokines. CCL21 has function in ininducing vigorous calcium migrations and chemotactic responses.

A DNA sequence encoding the human CCL21 (O00585) (Met 1-Pro 134) was expressed and purified.

6Ckine Protein, Human; CKb9 Protein, Human; ECL Protein, Human; MGC34555 Protein, Human; RP11-195F19.22-001 Protein, Human; SCYA21 Protein, Human; SLC Protein, Human; TCA4 Protein, Human

Chemokine (C-C motif) ligand 21

O00585

Baculovirus-Insect Cells

No tag

Neuroinflammation

< 1.0 EU per μg of the protein as determined by the LAL method.

> 98 % as determined by SDS-PAGE.

Measured by its binding ability in a functional ELISA. Immobilized human CCL21 at 2 μg/ml (100 μl/well) can bind human IGFBP7 with a linear range of 0.16-4 μg/ml.

Lyophilized

Lyophilized from sterile 40mM Tris, 0.3 M NaCl, pH 7.4 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Cytokine Proteins, Growth Factor proteins, Biological Target proteins, Immunotherapy proteins

The secreted recombinant human CCL21 consists of 111 amino acids and predicts a molecular mass of 12.3 kDa. It migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

CCL21

Human

https://www.uniprot.org/uniprot/O00585