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B7-1 Protein, Human, Recombinant

CAT: 0882-PH50277M0Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0882-PH50277M0Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
Overview
The mature form of human B7-1 (NP_005182.1) extracellular domain (Met 1-Asn 242) with five amini acids (DDDDK) at the C-terminus was expressed and purified.
Synonyms
B7 Protein, Human; B7-1 Protein, Human; B7.1 Protein, Human; BB1 Protein, Human; CD28LG Protein, Human; CD28LG1 Protein, Human; LAB7 Protein, Human
Gene Name
CD80 molecule
Expression System
HEK293 Cells
Tag
No tag
Field of Research
Cancer Drug Targets
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 85 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
B7 Family proteins, CD Molecule proteins, Cancer proteins, Immunotherapy proteins, Immune Checkpoint Proteins, Biological Target proteins
Molecular Weight
The mature recombinant human B7-1 consists of 214 amino acids and predicts a molecular mass of 25.5 kDa. As a result of glycosylation, rhB7-1 migrates as approximately 45 kDa band in SDS-PAGE.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
CD80
Species
Human
Protein ID
NP_005182.1
AA Sequence
Met1-Asn242
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/NP_005182.1

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