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Human UNC5B (Netrin Receptor UNC5B) Microsample ELISA Kit

CAT: 0855-ELK9758MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK9758MS-01Size:48 Tests
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Product Name Alternative
Netrin receptor UNC5B; Protein unc-5 homolog 2; Protein unc-5 homolog B; p53-regulated receptor for death and life protein 1; p53RDL1; UNC5B; P53RDL1; UNC5H2
UniProt
Q8IZJ1
Reactivity
Human
Field of Research
Neuroscience; Cardiovascular
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human UNC5B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human UNC5B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human UNC5B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human UNC5B in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.053 ng/mL

UniProtKB · Q8IZJ1

Netrin receptor UNC5B

UNC5B_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q8IZJ1
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
UNC5B
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q5T3R9, Q5T3S0, Q86SN3, Q8N1Y2, Q9H9F3
Protein keywords

Coding sequence diversity

Alternative splicing

Biological process

AngiogenesisApoptosis

Cellular component

Cell membraneMembrane

Molecular function

Developmental proteinReceptor

PTM

Disulfide bondGlycoproteinLipoproteinPalmitatePhosphoprotein

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix

Technical term

Proteomics identificationReference proteome

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