Human LEPR (Leptin Receptor) Microsample ELISA Kit

CAT: 0855-ELK10392MS-01Size: 48 TestsDry Ice: NoHazardous: No

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CAT#:0855-ELK10392MS-01Size:48 Tests

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Product Name Alternative

CD295; LEPR; OBR; LR

UniProt

P48357

Reactivity

Human

Field of Research

Signal transduction; CD & Adhesion molecule; Metabolic pathway; Endocrinology

Assay Type

Sandwich

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LEPR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LEPR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LEPR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LEPR in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

3.5h

Standard

200 ng/mL

Sample Type

Serum, plasma, tissue homogenates and other biological fluids

Detection Range

3.13-200 ng/mL

Sensitivity

1.11 ng/mL

UniProtKB · P48357

Leptin receptor

LEPR_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P48357
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: LEPR
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: LEP-R
EC number: —
Processing: Precursor
Secondary accessions: Q13592, Q13593, Q13594, Q92919, Q92920, Q92921

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembraneSecreted

PTM

Disulfide bondGlycoproteinPhosphoprotein

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix

Disease

Obesity

Molecular function

Receptor

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