Rat AT (Antithrombin) Microsample ELISA Kit

CAT: 0855-ELK7355MS-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK7355MS-01Size:48 Tests
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Product Name Alternative
AT3; ATIII; SERPINC1; Anti-Thrombin Antibodies; Serpin Peptidase Inhibitor Clade C Member 1; Coding Sequence Signal Peptide Antithrombin Part 1
Reactivity
Rat
Field of Research
Enzyme & Kinase; Metabolic pathway; Hematology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AT in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 ng/mL
Sample Type
Plasma
Detection Range
15.63-1000 ng/mL
Sensitivity
7 ng/mL

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