Rat RNLS (Renalase) Microsample ELISA Kit

CAT: 0855-ELK6942MS-01Size: 48 TestsDry Ice: NoHazardous: No

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CAT#:0855-ELK6942MS-01Size:48 Tests

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Product Name Alternative

MAO-C; C10orf59; FAD-Dependent Amine Oxidase; Monoamine oxidase-C; alpha-NAD (P) H oxidase/anomerase

UniProt

Q5U2W9

Reactivity

Rat

Field of Research

Enzyme & Kinase

Assay Type

Sandwich

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat RNLS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat RNLS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat RNLS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat RNLS in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

3.5h

Standard

100 ng/mL

Sample Type

Serum, plasma and other biological fluids

Detection Range

1.57-100 ng/mL

Sensitivity

0.69 ng/mL

UniProtKB · Q5U2W9

Renalase

RNLS_RAT · Rattus norvegicus

View on UniProt ↗

Primary accession: Q5U2W9
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: Rnls
Protein existence: 2: Evidence at transcript level
Organism: Rattus norvegicus (Rat)
Taxonomy ID: 10116
Alternative names: —
EC number: 1.6.3.5
Processing: Precursor
Secondary accessions: —

Protein keywords

Ligand

FADFlavoproteinNADNADP

Molecular function

Oxidoreductase

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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