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Human AT (Antithrombin) Microsample ELISA Kit

CAT: 0013-GTR18873223-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873223-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Human AT (Antithrombin) Microsample ELISA Kit
Product Name Alternative
AT3; ATIII; SERPINC1; Anti-Thrombin Antibodies; Serpin Peptidase Inhibitor Clade C Member 1; Coding Sequence Signal Peptide Antithrombin Part 1
Reactivity
Human
Field of Research
Hematology, Metabolism Research, Protein Biochemistry
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
1.49 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AT in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
3.13-200 ng/mL

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