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Human NFkB2 (Nuclear Factor Kappa B2) Microsample ELISA Kit

CAT: 0855-ELK4392MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4392MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
LYT-10; H2TF1; Lymphocyte translocation chromosome 10; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 2 (p49/P100) ; Nuclear factor NF-kappa-B p52
UniProt
Q00653
Reactivity
Human
Field of Research
Signal transduction
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NFkB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NFkB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NFkB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NFkB2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.32-20 ng/mL
Sensitivity
0.13 ng/mL

UniProtKB · Q00653

Nuclear factor NF-kappa-B p100 subunit

NFKB2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q00653
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
NFKB2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A8K9D9, D3DR83, Q04860, Q9BU75, Q9H471, Q9H472
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Molecular function

ActivatorDNA-bindingRepressor

Coding sequence diversity

Alternative splicingChromosomal rearrangement

Domain

ANK repeatRepeat

Biological process

Biological rhythmsTranscriptionTranscription regulation

Cellular component

CytoplasmNucleus

Disease

Disease variantProto-oncogene

PTM

Isopeptide bondPhosphoproteinUbl conjugation

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