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Mouse GAL12 (Galectin 12) Microsample ELISA Kit

CAT: 0855-ELK2900MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2900MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
LGALS12; GRIP1; Lectin, Galactoside-Binding Soluble 12; Galectin-related inhibitor of proliferation
UniProt
Q91VD1
Reactivity
Mouse
Field of Research
Metabolic pathway
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GAL12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GAL12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GAL12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GAL12 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
15.63-1000 pg/mL
Sensitivity
6.2 pg/mL

UniProtKB · Q91VD1

Galectin-12

LEG12_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q91VD1
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Lgals12
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
Gal-12
EC number
—
Processing
—
Secondary accessions
Q3UW90, Q8CCA4, Q8K2L7, Q9JKX2
Protein keywords

Coding sequence diversity

Alternative splicing

Biological process

Apoptosis

Ligand

Lectin

Cellular component

Nucleus

Technical term

Reference proteome

Domain

Repeat

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