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Human GAL12 (Galectin 12) Microsample ELISA Kit

CAT: 0855-ELK5304MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK5304MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
LGALS12; GRIP1; Lectin, Galactoside-Binding Soluble 12; Galectin-related inhibitor of proliferation
UniProt
Q96DT0
Reactivity
Human
Field of Research
Metabolic pathway
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GAL12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GAL12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GAL12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GAL12 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
2000 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
31.25-2000 pg/mL
Sensitivity
13.1 pg/mL

UniProtKB · Q96DT0

Galectin-12

LEG12_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q96DT0
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
LGALS12
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Gal-12
EC number
—
Processing
—
Secondary accessions
B2R9N2, G5E970, Q96DS9, Q96PR9, Q9H258, Q9H259, Q9NZ02
Protein keywords

Coding sequence diversity

Alternative splicing

Biological process

Apoptosis

Ligand

Lectin

Cellular component

Nucleus

Technical term

Proteomics identificationReference proteome

Domain

Repeat

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