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Mouse GAL12 (Galectin 12) Microsample ELISA Kit

CAT: 0013-GTR18872788-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872788-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mouse GAL12 (Galectin 12) Microsample ELISA Kit
Product Name Alternative
LGALS12; GRIP1; Lectin, Galactoside-Binding Soluble 12; Galectin-related inhibitor of proliferation
UniProt
Q91VD1
Reactivity
Mouse
Field of Research
Cell Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
6.2 pg/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GAL12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GAL12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GAL12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GAL12 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
15.63-1000 pg/mL

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