Human ET-1 (Endothelin1) Microsample ELISA Kit

CAT: 0855-ELK2444MS-01Size: 48 TestsDry Ice: NoHazardous: No

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CAT#:0855-ELK2444MS-01Size:48 Tests

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Product Name Alternative

ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1

UniProt

P05305

Reactivity

Human

Field of Research

Endocrinology; Cardiovascular biology

Assay Type

Sandwich

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

3.5h

Standard

500 pg/mL

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection Range

7.82-500 pg/mL

Sensitivity

2.69 pg/mL

UniProtKB · P05305

Endothelin-1

EDN1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P05305
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: EDN1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: Q96DA1

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

Cleavage on pair of basic residuesDisulfide bond

Disease

Disease variant

Cellular component

Secreted

Domain

Signal

Molecular function

VasoactiveVasoconstrictor

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