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Human ET-1 (Endothelin1) Microsample ELISA Kit

CAT: 0013-GTR18872952-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872952-01Size:48 Tests
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Description
Human ET-1 (Endothelin1) Microsample ELISA Kit
Product Name Alternative
ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1
UniProt
P05305
Reactivity
Human
Field of Research
Cardiovascular Research, Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
2.69 pg/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
7.82-500 pg/mL

UniProtKB · P05305

Endothelin-1

EDN1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P05305
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
EDN1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q96DA1
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

Cleavage on pair of basic residuesDisulfide bond

Disease

Disease variant

Cellular component

Secreted

Domain

Signal

Molecular function

VasoactiveVasoconstrictor

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