Dog ET-1 (Endothelin1) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1

• Gene Aliases:

  ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1

• UniProt:

  P13206

• Reactivity:

  Dog

• Field of Research:

  Endocrinology, Cardiovascular biology

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  500 pg/mL

• Sample Type:

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

• Detection Range:

  7.82 - 500 pg/mL

• Sensitivity:

  2.65 pg/mL

• Species:

  Dog

• CAS Number:

  7732-18-5