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Human PRF1 (Perforin 1) Microsample ELISA Kit

CAT: 0855-ELK1879MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1879MS-01Size:48 Tests
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Product Name Alternative
FLH2; HPLH2; P1; PFP; Pore Forming Protein; Cytolysin; Lymphocyte pore-forming protein
UniProt
P14222
Reactivity
Human
Field of Research
Immune molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PRF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PRF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PRF1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
1.57-100 ng/mL
Sensitivity
0.64 ng/mL

UniProtKB · P14222

Perforin-1

PERF_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P14222
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PRF1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
P1
EC number
—
Processing
Precursor
Secondary accessions
B2R6X4, Q59F57, Q86WX7
Protein keywords

Ligand

CalciumMetal-binding

Cellular component

Cell membraneEndosomeLysosomeMembraneSecreted

Biological process

Cytolysis

Disease

Disease variantFamilial hemophagocytic lymphohistiocytosis

PTM

Disulfide bondGlycoprotein

Domain

EGF-like domainSignalTransmembraneTransmembrane beta strand

Technical term

Proteomics identificationReference proteome

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