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Mouse PRF1 (Perforin 1) Microsample ELISA Kit

CAT: 0855-ELK3068MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3068MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
FLH2; HPLH2; P1; PFP; Pore Forming Protein; Cytolysin; Lymphocyte pore-forming protein
UniProt
Q42449
Reactivity
Mouse
Field of Research
Immune molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PRF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PRF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PRF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PRF1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.064 ng/mL

UniProtKB · Q42449

Profilin-1

PRF1_ARATH · Arabidopsis thaliana

View on UniProt ↗
Primary accession
Q42449
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PRF1
Protein existence
1: Evidence at protein level
Organism
Arabidopsis thaliana (Mouse-ear cress)
Taxonomy ID
3702
Alternative names
—
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Technical term

3D-structureReference proteome

Molecular function

Actin-binding

Disease

Allergen

Cellular component

CytoplasmCytoskeleton

Biological process

Plant defense

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