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Rat PRF1 (Perforin 1) Microsample ELISA Kit

CAT: 0013-GTR18871950-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18871950-01Size:48 Tests
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Description
Rat PRF1 (Perforin 1) Microsample ELISA Kit
Product Name Alternative
FLH2; HPLH2; P1; PFP; Pore Forming Protein; Cytolysin; Lymphocyte pore-forming protein
UniProt
P35763
Reactivity
Rat
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Sensitivity
0.06 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PRF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PRF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PRF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PRF1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · P35763

Perforin-1

PERF_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P35763
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Prf1
Protein existence
2: Evidence at transcript level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
P1
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Ligand

CalciumMetal-binding

Cellular component

Cell membraneEndosomeLysosomeMembraneSecreted

Biological process

Cytolysis

PTM

Disulfide bondGlycoprotein

Domain

EGF-like domainSignalTransmembraneTransmembrane beta strand

Technical term

Reference proteome

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