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Human VNN1 (Vanin 1) Microsample ELISA Kit

CAT: 0855-ELK3314MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3314MS-01Size:48 Tests
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Product Name Alternative
Pantetheinase; Pantetheine hydrolase; Tiff66; Vascular non-inflammatory molecule 1
UniProt
O95497
Reactivity
Human
Field of Research
Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VNN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VNN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VNN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VNN1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.053 ng/mL

UniProtKB · O95497

Pantetheinase

VNN1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O95497
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
VNN1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.5.1.92
Processing
Precursor
Secondary accessions
A8K310, Q4JFW6, Q4VAS7, Q4VAS8, Q4VAS9, Q9UF16, Q9UJF4
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Cellular component

Cell membraneMembrane

PTM

GlycoproteinGPI-anchorLipoprotein

Molecular function

Hydrolase

Domain

Signal

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