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Human IL20 (Interleukin 20) Microsample ELISA Kit

CAT: 0855-ELK1291MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1291MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
IL-20; IL10D; IL10-D; ZCYTO10; Cytokine Zcyto10
UniProt
Q9NYY1
Reactivity
Human
Field of Research
Cytokine; Infection immunity; Dermatology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL20. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL20. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL20 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range
15.63-1000 pg/mL
Sensitivity
6 pg/mL

UniProtKB · Q9NYY1

Interleukin-20

IL20_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9NYY1
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IL20
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IL-20
EC number
—
Processing
Precursor
Secondary accessions
Q17RB3, Q2THG6, Q96QZ6
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Molecular function

Cytokine

PTM

Disulfide bond

Cellular component

Secreted

Domain

Signal

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