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Human IL29 (Interleukin 29) Microsample ELISA Kit

CAT: 0855-ELK1784MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1784MS-01Size:48 Tests
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Product Name Alternative
IL-29; FNL1; Interferon, Lambda 1; Cytokine Zcyto21
UniProt
Q8IU54
Reactivity
Human
Field of Research
Cytokine; Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL29. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL29. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL29, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL29 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
500 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
7.82-500 pg/mL
Sensitivity
2.6 pg/mL

UniProtKB · Q8IU54

Interferon lambda-1

IFNL1_HUMAN · Homo sapiens

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Primary accession
Q8IU54
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IFNL1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IFN-lambda-1
EC number
—
Processing
Precursor
Secondary accessions
A0AV25, Q17R34
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Biological process

Antiviral defense

Molecular function

Cytokine

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

Domain

Signal