Human IL22 (Interleukin 22) Microsample ELISA Kit

CAT: 0855-ELK2748MS-01Size: 48 TestsDry Ice: NoHazardous: No

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CAT#:0855-ELK2748MS-01Size:48 Tests

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Product Name Alternative

IL-22; ILTIF; Zcyto18; IL-TIF; IL-D110; TIFa; TIFIL-23; Cytokine Zcyto18; IL-10-Related T-Cell-Derived Inducible Factor

UniProt

Q9GZX6

Reactivity

Human

Field of Research

Cytokine; Infection immunity; Pulmonology; Gastroenterology

Assay Type

Sandwich

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL22. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL22. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL22, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL22 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

3.5h

Standard

1000 pg/mL

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection Range

15.63-1000 pg/mL

Sensitivity

5.8 pg/mL

UniProtKB · Q9GZX6

Interleukin-22

IL22_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: Q9GZX6
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: IL22
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: IL-22
EC number: —
Processing: Precursor
Secondary accessions: —

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Molecular function

Cytokine

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

Domain

Signal

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